BIOTA Philippines Conference System, 53rd BIOTA Annual National Convention and Scientific Sessions

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EVALUATION OF THE ANTIPLASMODIAL PROPERTY OF THE CHROMATOGRAPHIC FRACTIONS OF AZADIRACHTA EXCELSA DICHLOROMETHANE LEAF SUBEXTRACTS ON IN VITRO CULTURE SYSTEMS OF PLASMODIUM FALCIPARUM
Janina Erika Galang Sese, James Darryl Pajes Bequin, Terence Macaraig Lapeñas, Christine Joyce Beloso Prado, Andrea Betina Montes Vega, Edilberto Dela Paz Manahan, Oliver Villaflores, Kulachart Jangpatarapongsa

Last modified: 2018-03-17

Abstract


Malaria is a life-threatening disease caused by Plasmodium parasites. There were 212 million new cases of malaria worldwide in 2015 alone. (WHO, 2016) Azadirachta excelsa also known as Philippine Neem tree is a native plant found in the Philippines with several medicinal applications. The study aims to know which among the semi-polar subextracts of Azadirachta excelsa exhibits the greatest antiplasmodial property against in vitro cultures of blood-stage Plasmodium falciparum NF54 strain by measuring the decrease in the level of parasitemia. Azadirachta excelsa DCM crude fractions were subjected to further fractionation through VLC. The subextracts were pooled using TLC and purified through ROTAVAP. They were subjected to malarial culture at two time intervals at different concentrations with positive, negative and solvent controls. Giemsa-stained smears were prepared and read microscopically. Each stage of the malarial parasite seen in 1000 red blood cells were counted to obtain the level of parasitemia. A phytochemical screening of the best fraction was performed using TLC for profiling and tested with spray reagents. Analysis of data showed that Azadirachta excelsa had a significant effect at higher concentrations against in vitro culture systems of Plasmodium falciparum parasites. All of the seven extracts have shown a significant difference from the negative and solvent control setup. Mechanism of antiplasmodial activity of the plant extract is through killing of malarial parasite, as level of percent parasitemia decreased over time and eventually reached 0.3% at 50 ug/mL concentration for Fraction 3. It is therefore concluded that Fraction 3 showed the best antiplasmodial activity. The antiplasmodial activity can be attributed to the presence of the phytochemicals present in the fraction namely Flavonoids, Steroids, Phenols, Tannins, Cardenolides, Coumarin, Anthraquinones, Anthrones, Indole, Higher Alcohols, and Sugars.


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