BIOTA Philippines Conference System, 49th BIOTA Annual National Convention and Scientific Sessions

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105A/C IL-18 GENE SINGLE NUCLEOTIDE POLYMORPHISM AND HOUSE DUST MITE ALLERGY IN A FILIPINO POPULATION
Eisha Vienna Maliksi Fernandez, Cristan Q. Cabanilla, Hiyasmin Lim, John Donnie A. Ramos

Last modified: 2014-02-20

Abstract


Allergy is a multifactorial disease affecting a significant proportion of the population. It is developed through the interaction of environment allergens and the presence of susceptibility genes.A single nucleotide polymorphism is a genetic variation that features a difference around a single base of a gene, where analternative form may predispose an individual to a certain disease. In this study, the correlation of the 105A/C single nucleotide polymorphism (SNP) of the IL-18 gene and house dust mite-specific IgE among Filipino allergic and non-allergic population was investigated. Two hundred twenty match-paired cases and controls aged 6-60 were the subjects of this investigation. Recruitment of participants was conducted in Bacoor City Health Centers, Philippine Normal University, and Philippine Childrens Medical Center. The level of total IgE and Specific IgE were measured using enzyme-linked immunosorbent assay (ELISA).Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was used in screening for the 105A/C SNP of IL-18.Sensitization profiles of the allergic patients revealed that 88.2% were sensitized to Dermatophagoides farinae, 82.7% to Dermatophagoides pteronyssinus, and 10.9% to Blomia tropicalis. Results show that the allergic participants were distinguished from their non-allergic counterparts by their allelic frequency. Initial findings show that there is an overrepresentation of the A allele in the atopic participants, and a greater prevalence of A/A homozygotes compared to the A/C and C/C. This indicates that the IL-18 gene polymorphism is a candidate genetic marker for allergy.


Keywords


house dust mite allergy; interleukin-18 (IL-18); single nucleotide polymorphism

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